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RNA Interference (RNAi): Targeted Gene Silencing RNAi has emerged as an important mainstream tool for both basic- and applied research, and it has revolutionized the novel discipline known as functional genomics. RNAi is ubiquitous in eukaryotes and currently is the preferred tool for investigating the regulation of gene expression in plants, animals, and fungi. This method is becoming increasingly popular because of its wide-ranging applicability in research, which results from compatibility with cell culture and in vivo models. Comparatively speaking, RNAi is much faster and more economical than creating knock-out animals in order to study the function of specific genes. The resulting precision silencing of specific genes also makes RNAi an attractive platform for the discovery and development of life-saving pharmaceuticals. Continuing Education Units (CEUs) will be awarded upon completion of this workshop.
► Overview of RNAi, including Essential RNAi Vocabulary ► Short Interfering RNA Approaches (siRNA) ► Short Hairpin RNA Approaches (shRNA) ► Micro-RNA Approaches (miRNA) ► DNA-Directed RNA Interference ► “Take a RISC – Role the Dicer” ► Design Strategies ► siRNA Delivery Methods ► Proper Handling of RNA and the RNase Peril ► Applications for RNAi ► RNA, Protein, and Functional Evaluation Assays Laboratory Topics: ► Working with Pools of siRNA ► Cell Transfection ► Determining Efficiency of RNAi ► RT-PCR and Blot Analysis ► Western Blot Analysis Dates / Locations / Tuition This workshop will be conducted on-site only (at your location) in 2011. Please contact us for details. Exon-Intron, Inc. |