DNA Typing
RFLP & PCR-based Systems

Slight but unique differences in the banding pattern of DNA fragments from different individuals of a species are observed when subjected to restriction enzyme analysis. These variations in the DNA are called restriction fragment length polymorphisms (RFLPs) and are used as markers on both physical and genetic linkage maps. Such differences in the RFLP profiles have revolutionized criminal investigations and have become powerful tools in the identification of individuals in paternity and maternity cases, population genetics, and in the diagnosis of a variety of diseases.

More recently, polymerase chain reaction (PCR)-based tests have been used to obtain a DNA profile. Such tests are generally less involved, providing rapid results that can serve as an alternative or as a complement to RFLP tests.

This integrated laboratory and lecture course is designed to provide the participant with a basic understanding of the principles and methodologies used in RFLP and PCR-based analyses. The practical applications of RFLP analysis, coupled with the use of PCR-amplified products the VNTR locus D1S80, are emphasized in selected topics for the forensic and research scientist as well as for those in the legal community. Continuing Education Units (CEUs) available.