Recombinant DNA Methodology

This integrated laboratory and lecture course is designed to provide a solid background in standard molecular biology techniques. The course emphasizes the practical application of rDNA technology with clear explanations of the rationale behind differing approaches. The extensive laboratory portion of the course affords the participant an opportunity to perform a variety of classical and cutting-edge molecular techniques. Continuing Education Units (CEUs) available.



Topics

         Introduction to rDNA - Overview of Methods and Applications

         Nucleic Acid Organization and Structure

         Isolation of Genomic DNA

         Isolation of RNA

         Selection of Poly(A)+ RNA by Magnetic Bead Technology

         Northern Analysis

         Isolation of Plasmid DNA

         Restriction Endonuclease Digestion of Plasmid and Genomic DNA

         Agarose Gel Electrophoresis

         Southern Analysis

         DNA Vector-Insert Ligation

         Strategies for the Synthesis and Cloning of cDNA

         Bacterial Transformation

         Cloning into Lambda Bacteriophage

         Antibody Screening of Lambda Libraries

         Non-isotopic Probe Synthesis and Detection by Chemiluminescence

         Introduction to PCR

 

Dates / Locations / Tuition

This workshop will be conducted on-site only (at your location) in  2011. Please contact us (workshop@DNAtech.com) for details.

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Exon-Intron, Inc.
P.O. Box 395
Loganville, PA 17342
Tel: 717-428-0270
Fax: 717-771-4062
workshop@DNAtech.com