Polymerase Chain Reaction Methodology

This integrated laboratory and lecture course is designed to provide a solid understanding of the parameters which influence PCR. Once the basics have been learned, PCR is then used to mutagenize one of the PCR products. The PCR product is cloned and analyzed using popular blue/white selection methods, and validated by PCR performed on individual clones. Lab work includes a variety of PCR reactions and ancillary cloning techniques. Continuing Education Units (CEUs) available.


Topics

         PCR: An Overview

         Components and Conditions for PCR Optimization

         Primer Design

         Alternative Enzyme Platforms

         Isolation of Nucleic Acids for PCR Amplification

         Site-Directed Mutagenesis by PCR

         Restriction Enzyme Analysis of PCR Products

         Cloning of PCR Products

         Symmetric PCR

         Asymmetric PCR

         Inverse PCR

         Anchored PCR

         RT-PCR

         RT-PCR Applications

         Real-Time PCR (limited hands-on)

         Research Applications of PCR

         Non-isotopic Probe Synthesis and Detection by Chemiluminescence

 

 

Date / Location / Tuition

May 16 - 20, 2011 / Penn State York, York, PA / $1550.00

This workshop can also be conducted at YOUR location.  Please contact us for details.

Related Course

Advanced PCR Techniques

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Exon-Intron, Inc.
P.O. Box 395
Loganville, PA 17342
Tel: 717-428-0270
Fax: 717-771-4062
workshop@DNAtech.com