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RNA Methodologies: A Laboratory Guide for Isolation and Characterization (4th edition, 2010)
By Exon-Intron's Dr. Robert Farrell


This is the fourth edition of the successful laboratory guide which has translated the rich story of RNA for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of animal and plant RNA, with lesser emphasis on prokaryotic systems. This lab guide emphasizes transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively, the chapters all work together providing analysis with clear take-home lessons to assist researchers to understand the subtleties of working with RNA and to optimize time at the bench. The abundant use of flow charts, tables, and the inclusion of representative data are especially helpful in the planning and implementation phases of a project and facilitate learning. The 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, a new chapter dealing with the molecular biology of plants, and an expanded glossary. The Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational center.



Molecules of Life and Mutations: Understanding Diseases by Understanding Proteins (2002)
by Siegfried Schwarz

This book provides insights into the structures and functions of 130 of the most important biomolecules and their interactions with other endogenous or exogenous molecules. These interactions are illustrated by 3-dimensional images of their atomic structures rather than by abstract formulas or acronyms. The Author has compiled an extraordinary collection of molecules which he has visualized in pictures of stunning clarity and beauty by applying molecular modeling software to their atomic coordinate files (deposited in the Brookhaven Protein Data Bank, PDB). Together with short explanatory texts they provide the reader with a deepened understanding of biological phenomena in the normal as well as the diseased organism.




PCR Primer: A Laboratory Manual (2nd edition, 2003) 
Carl W. Dieffenbach and Gabriela S. Dveksler (Editors)

A guide to the complexities of the polymerase chain reaction that begins at an accessible level and then moves to more advanced levels of application. First, the practical requirements for performing PCR and other amplification techniques in the lab are introduced, and then the basic aspects of the technique are explained by exploring issues such as sample preparation, primer design, efficiency, detection of products, and quantitation.

Protocols for a wide range of PCR and amplification techniques are presented for cloning, sequencing, mutagenesis, library construction and screeing, exon trapping, differential display, and expression; these include RT-PCR, RNA PCR, LCR, multiplex PCR, panhandle PCR, capture PCR, expression PCR, 3′ and 5′ RACE, in situ PCR, and ligation-mediated PCR.








Recombinant DNA: Genes and Genomes - A Short Course (3rd edition, 2006)
James D. Watson, Richard M. Myers, Amy Caudy, and Jan A. Witkowski

The third edition of the landmark text “Recombinant DNA” offers an authoritative, accessible, and engaging introduction to modern, genome centered biology from its foremost practitioners. The new edition explores core concepts in molecular biology in a contemporary inquiry based context, building its coverage around the most relevant and exciting examples of current research and landmark experiments that redefined our understanding of DNA. As a result, students learn in a compelling way how working scientists make real high impact discoveries. The first chapters provide an introduction to the fundamental concepts of genetics and genomics, an inside look at the Human Genome Project, bioinformatic and experimental techniques for large scale genomic studies, and a survey of epigenetics and RNA interference. The final chapters cover the quest to identify disease causing genes, the genetic basis of cancer, and DNA fingerprinting and forensics. In these chapters the authors provide examples of practical applications in human medicine, and discuss the future of human genetics and genomics projects.






Genes X (2010)

The field of molecular biology and molecular genetics is rapidly changing with new data acquired daily and new insights into well-studied processes presented on a scale of weeks or months rather than years. For decades Lewin's GENES has provided the teaching community with the most cutting edge presentation of molecular biology and molecular genetics, covering gene structure, sequencing, organization, and expression. The latest edition, with a knowledgeable new author team, has enlisted 21 scientists to provide revisions and content updates in their individual fields of expertise, ensuring that Lewin's GENES X is the most current and comprehensive text in the field. Informative new chapters, as well as a reorganization of material, provide a more logical flow of topics and many chapters have been renamed to better indicate their contents. Lewin's GENES X also contains new pedagogical features to help students learn as they read and an online student study guide allows students to test themselves on key material.

Molecular Cloning: A Laboratory Manual

(3 Volume Set; 3rd edition, 2001)
by Joseph Sambrook and David W. Russell


“In every kitchen there is at least one indispensable cookbook. Sambrook and Russell's Molecular Cloning: A Laboratory Manual fills the same niche in the laboratory. Like its kitchen counterparts, Sambrook’s Molecular Cloning has information to help both the inexperienced and the advanced user.” --Trends in Neurosciences




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